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Johns Hopkins HealthCare deep sequencing and microarray core facility
Deep Sequencing And Microarray Core Facility, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deep+sequencing+and+microarray+core+facility/deep+sequencing+and+microarray+core/pm39341835-495-13-6
Average 90 stars, based on 1 article reviews
deep sequencing and microarray core facility - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Sequencing:

Article Title: Type II alveolar epithelial cell–specific loss of RhoA exacerbates allergic airway inflammation through SLC26A4
Article Snippet: .. 13 6JHMI Deep Sequencing and Microarray Core Facility, Johns Hopkins University School of Medicine, 14 Baltimore, MD 21224, USA. ..

Article Title: Replication Study: Transcriptional amplification in tumor cells with elevated c-Myc
Article Snippet: .. L Michelle Lewis, Meredith C Edwards, Zachary R Meyers, David Blum: Bioexpression and Fermentation Facility, University of Georgia is a Science Exchange associated lab. C Conover Talbot Jr: Deep Sequencing and Microarray Core Facility, Johns Hopkins University is a Science Exchange associated lab. Reproducibility Project: Cancer Biology: EI, RT, NP: Employed by and hold shares in Science Exchange Inc. ..

Article Title: Endotoxemia-mediated activation of acetyltransferase P300 impairs insulin signaling in obesity
Article Snippet: .. Microarray analysis was conducted in the Johns Hopkins Deep Sequencing and Microarray Core Facility. .. A far-western blot was conducted using a modified method of Wu et al. Purified IRS1/2-WT protein or their mutants were separated using a NuPAGETM NovexTM Tris-Acetate Protein Gel (Thermo Fisher Scientific) and transferred onto a polyvinylidene fluoride membrane.

Article Title: Replication Study: Transcriptional amplification in tumor cells with elevated c-Myc
Article Snippet: .. Deep Sequencing and Microarray Core Facility, Johns Hopkins University is a Science Exchange associated lab. ..

Article Title: Methylated cis-regulatory elements mediate KLF4-dependent gene transactivation and cell migration
Article Snippet: Teton KLF4 WT and R458A cells were treated with Dox for 48 hr followed by ChIP using the anti-KLF4 antibody and Dynabeads Protein A/G (Thermo Fisher Scientific) according to a protocol described previously (Hu et al., 2013). .. DNA library construction and sequencing was performed at Johns Hopkins Deep Sequencing and Microarray Core Facility. .. The antibodies used for ChIP experiments were as follows: anti-KLF4 (Santa Cruz, H-180, sc-20691); anti-H3K27ac (Abcam, ab4279) (Hawkins et al., 2010); anti-H3K27me3 (Millipore, 07–449) (Hawkins et al., 2010) and anti-H3K9me3 (Abcam, ab8898) (Hawkins et al., 2010).

Article Title: Hypoxia induces ROS-resistant memory upon reoxygenation in vivo promoting metastasis in part via MUC1-C.
Article Snippet: We are grateful for being selected for the 2018 Core Coins grant program offered by the Johns Hopkins Experimental and Computational Genomics Core (ECGC). .. We thank Linda Orzolek of the Johns Hopkins Medical Institutions Deep Sequencing and Microarray Core Facility for technical assistance. ..

Microarray:

Article Title: Type II alveolar epithelial cell–specific loss of RhoA exacerbates allergic airway inflammation through SLC26A4
Article Snippet: .. 13 6JHMI Deep Sequencing and Microarray Core Facility, Johns Hopkins University School of Medicine, 14 Baltimore, MD 21224, USA. ..

Article Title: Replication Study: Transcriptional amplification in tumor cells with elevated c-Myc
Article Snippet: .. L Michelle Lewis, Meredith C Edwards, Zachary R Meyers, David Blum: Bioexpression and Fermentation Facility, University of Georgia is a Science Exchange associated lab. C Conover Talbot Jr: Deep Sequencing and Microarray Core Facility, Johns Hopkins University is a Science Exchange associated lab. Reproducibility Project: Cancer Biology: EI, RT, NP: Employed by and hold shares in Science Exchange Inc. ..

Article Title: Endotoxemia-mediated activation of acetyltransferase P300 impairs insulin signaling in obesity
Article Snippet: .. Microarray analysis was conducted in the Johns Hopkins Deep Sequencing and Microarray Core Facility. .. A far-western blot was conducted using a modified method of Wu et al. Purified IRS1/2-WT protein or their mutants were separated using a NuPAGETM NovexTM Tris-Acetate Protein Gel (Thermo Fisher Scientific) and transferred onto a polyvinylidene fluoride membrane.

Article Title: Replication Study: Transcriptional amplification in tumor cells with elevated c-Myc
Article Snippet: .. Deep Sequencing and Microarray Core Facility, Johns Hopkins University is a Science Exchange associated lab. ..

Article Title: Methylated cis-regulatory elements mediate KLF4-dependent gene transactivation and cell migration
Article Snippet: Teton KLF4 WT and R458A cells were treated with Dox for 48 hr followed by ChIP using the anti-KLF4 antibody and Dynabeads Protein A/G (Thermo Fisher Scientific) according to a protocol described previously (Hu et al., 2013). .. DNA library construction and sequencing was performed at Johns Hopkins Deep Sequencing and Microarray Core Facility. .. The antibodies used for ChIP experiments were as follows: anti-KLF4 (Santa Cruz, H-180, sc-20691); anti-H3K27ac (Abcam, ab4279) (Hawkins et al., 2010); anti-H3K27me3 (Millipore, 07–449) (Hawkins et al., 2010) and anti-H3K9me3 (Abcam, ab8898) (Hawkins et al., 2010).

Article Title: Hypoxia induces ROS-resistant memory upon reoxygenation in vivo promoting metastasis in part via MUC1-C.
Article Snippet: We are grateful for being selected for the 2018 Core Coins grant program offered by the Johns Hopkins Experimental and Computational Genomics Core (ECGC). .. We thank Linda Orzolek of the Johns Hopkins Medical Institutions Deep Sequencing and Microarray Core Facility for technical assistance. ..

other:

Article Title: Inflammatory Monocytes Recruited to the Liver within 24 Hours after Virus-Induced Inflammation Resemble Kupffer Cells but Are Functionally Distinct
Article Snippet: After staining to identify Kupffer cells and inflammatory monocytes as described above, cells were fixed with 2% formaldehyde for 1 h and sorted on a FACSAria SORP flow cytometer (BD Biosciences).

Article Title: TATA-Box Binding Protein O-GlcNAcylation at T114 regulates formation of the B-TFIID complex and is critical for metabolic gene regulation
Article Snippet: Cells were quenched and scraped in 1 ml of ice-cold methanol, transferred to 2 ml Eppendorf tubes, snap frozen in liquid nitrogen for 10 min, thawed on ice for 10 min, vortexed and centrifuged at 12,000 x g for 10 min at 4°C.



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